Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer?
نویسندگان
چکیده
We report the spontaneous generation of a cell-like morphology in an environment crowded with the polymers dextran and polyethylene glycol (PEG) in the presence of DNA. DNA molecules were selectively located in the interior of dextran-rich micro-droplets, when the composition of an aqueous two-phase system (ATPS) was near the critical condition of phase-segregation. The resulting micro-droplets could be controlled by the use of optical tweezers. As an example of laser manipulation, the dynamic fusion of two droplets is reported, which resembles the process of cell division in time-reverse. A hypothetical scenario for the emergence of a primitive cell with DNA is briefly discussed.
منابع مشابه
Interaction and micellar behavior of aqueous mixtures of surface active ionic liquid and cationic surfactant: experimental and theoretical studies
The interaction between an ionic liquid (1-dodecyl-3-methylimidazolium bromide or IL) and cationic surfactant (dodecyltrimethylammonium bromide (DTAB)) in aqueous solution has been investigated at various mole fractions and temperature 30 ˚C using experimental and theoretical methods. The critical micelle concentration (CMC) of pure components and their binary mixtures, mixed micellar compositi...
متن کاملNano-sized Amitriptyline (AT) imprinted polymer particles: Synthesis and characterization in Silicon oil
Amitriptyline hydrochloride is a highly permeable active pharmaceutical ingredient (API). The function of these drugs is to block the reuptake of the neurotransmitters, norepinephrine and serotonin in the central nervous system. The nano-sized Amitriptyline (AT) imprinted polymer particles were synthesized successfully. The nanoparticles were characterized by Fourier transform infrared spectros...
متن کاملNano-sized Amitriptyline (AT) imprinted polymer particles: Synthesis and characterization in Silicon oil
Amitriptyline hydrochloride is a highly permeable active pharmaceutical ingredient (API). The function of these drugs is to block the reuptake of the neurotransmitters, norepinephrine and serotonin in the central nervous system. The nano-sized Amitriptyline (AT) imprinted polymer particles were synthesized successfully. The nanoparticles were characterized by Fourier transform infrared spectros...
متن کاملDepletion forces drive polymer-like self-assembly in vibrofluidized granular materials.
Ranging from nano- to granular-scales, control of particle assembly can be achieved by limiting the available free space, for example by increasing the concentration of particles ("crowding") or through their restriction to 2D environments. It is unclear, however, if self-assembly principles governing thermally-equilibrated molecules can also apply to mechanically-excited macroscopic particles ...
متن کاملA New Solution for the Cyclic Multiple-Part Type Three-Machine Robotic Cell Problem based on the Particle Swarm Meta-heuristic
In this paper, we develop a new mathematical model for a cyclic multiple-part type threemachine robotic cell problem. In this robotic cell a robot is used for material handling. The objective is finding a part sequence to minimize the cycle time (i.e.; maximize the throughput) with assumption of known robot movement. The developed model is based on Petri nets and provides a new method to calcul...
متن کامل